THE QUALITY AND CYTOTOXICITY ASSESSMENT OF THE ACELLULAR HUMAN LIVER SCAFFOLD AFTER DECELLULARIZATION

Currently the technologies for the treatment of liver failure to create a “bioartificial liver” are actively being developed in a parallel with the organ transplantation. Such tissue-engineered constructs obtained by devitalization can preserve both the native structure and the three-dimensional architecture of the liver, ensuring biocompatibility for subsequent recellularization. However, it is important to maintain a balance between cell removal and the quality of the extracellular matrix, since excessive devitalization can lead to degradation of the scaffold microarchitecture. Thus, the aim of the study was to assess the quality of the human liver scaffold after machine decellularization process.

The developed algorithm for decellularization of the human liver using machine perfusion makes it possible to obtain an acellular matrix in a minimum time period, which meets the main quality control criteria (absence of a cellular component, minimal content of residual DNA in the tissue, preservation of the extracellular matrix, sterility, absence of a cytotoxic effect).

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