Influence of the type and style of fever, smoking status, degree of androgenetic alopecia on the phases of hair growth in military servicemen under inpatient treatment with a diagnosis of pneumonia

The article discusses the role of lipopolysaccharide (LPS) of gram-negative bacteria in platelet activation and vascular reactions. LPS interacts with the TLR4 receptor on platelets, enhancing their aggregation and production of reactive oxygen species (ROS), which promotes the release of factors involved in inflammation and coagulation. The mechanism of action involves phosphorylation of key signaling proteins (Akt, ERK1/2, PLA2) through the TLR4-PI3K-Akt-ERK1/2 pathway, dependent on ROS and thromboxane A₂. The effect of LPS is also manifested in a decrease in vascular relaxation and an increase in vascular permeability, and reactions to its concentration range from increased ROS production to increased levels of inflammatory mediators. ROS play an important role in signaling by activating the MAPK and NF-kB cascades, which promotes the expression of enzymes and cytokines that regulate inflammation and vascular function. ROS plays an important role as an intermediary in the mechanisms of inflammation and platelet activation under the action of LPS, as well as their effect on prostanoid synthesis and vascular reactions.
Most NSAIDs are active against PGHS-1 In LPS-stimulated whole blood, S-ibuprofen markedly reduces TXB(2) levels in the concentration range of 10 to 200 μg/ml and platelet aggregation in non-inflammatory and inflammatory fever.

1. Saluk-Juszczak, J. Endotoxins stimulate generation of superoxide radicals and lipid peroxidation in blood / J. Saluk-Juszczak, B. Wachowicz, W. Kaca // Microbios. 2000; 103(404): 17–25.

2. Maria, E. Lopes Pires, Lipopolysaccharide potentiates platelet responses via toll-like receptor 4-stimulated Akt-Erk-PLA2 signalling / E. Maria Lopes Pires, R. SimonClarke, Sisi Marcondes, Jonathan M Gibbins // PLoS One; 11.

3. Raquel, Hernanz, Hypertension alters role of iNOS, COX-2, and oxidative stress in bradykinin relaxation impairment after LPS in rat cerebral arteries / Raquel Hernanz, Ana M Briones, María J Alonso, Elisabet Vila, Mercedes Salaices // Am J Physiol Heart Circ Physiol . 2004 Jul;287(1)

4. Alejandro, M. S. Mayer, Vibrio vulnificus MO6-24 / O lipopolysaccharide stimulates superoxide anion, thromboxane B 2, matrix metalloproteinase-9, cytokine and chemokine release by rat brain microglia in vitro / Alejandro M. S. Mayer, Mary L. Hall, Michael Holland, Cristina De Castro, Antonio Molinaro, Monica Aldulescu, Jeffrey Frenkel, Lauren Ottenhoff, David Rowley, Jan Powell // Mar Drugs. 2014 Mar 26;12(4).

5. Oketani, K. Effect of E3040, an inhibitor of 5-lipoxygenase and thromboxane synthase, on rat bowel damage induced by lipopolysaccharide / K. Oketani, T. Inoue, M. Murakami // Eur J Pharmacol. 2001 Sep 14;427(2).

6. Feuerstein, N. Leukotrienes C4 and D4 induce prostaglandin and thromboxane release from rat peritoneal macrophages / N. Feuerstein, M. Foegh, P W Ramwell // Br J Pharmacol. 1981 Mar;72(3):389–91.

7. Alejandro, M. S. Mayer, Cyanobacterial Microcystis aeruginosa lipopolysaccharide elicits release of superoxide anion, thromboxane B 2, cytokines, chemokines, and matrix metalloproteinase-9 by rat microglia / Alejandro M S Mayer, Jonathan A Clifford, Monica Aldulescu, Jeffrey A Frenkel, Michael A Holland, Mary L Hall, Keith B Glaser, John Berry // Toxicol Sci . 2011 May;121(1):63–72

8. Korbecki, J. The effect of reactive oxygen species on the synthesis of prostanoids from arachidonic acid / J. Korbecki, I. Baranowska-Bosiacka, I. Gutowska, D Chlubek // J Physiol Pharmacol . 2013 Aug;64(4):409–21.

9. Винокуров, М. Г. Исследование механизмов действия липополисахарида Rhodobacter capsulatus на функциональные ответы и апоптоз фагоцитов крови человека при активации эндотоксинами / М. Г. Винокуров. – Москва, 2007. – 49 с. – Автореферат диссертации на соискание ученой степени доктора биологических наук.

10. Seethanathan, P. Characterization of release of tumor necrosis factor, interleukin-1, and superoxide anion from equine white blood cells in response to endotoxin / P. Seethanathan, G. D. Bottoms, K. Schafer // Am J Vet Res. 1990 Aug;51(8):1221–5.

11. Carlos Jiménez-Romero Dactyloditerpenol acetate, a new prenylbisabolane-type diterpene from Aplysia dactylomela with significant in vitro anti-neuroinflammatory activity / Carlos Jiménez-Romero, Alejandro M S Mayer, Abimael D Rodríguez // Bioorg Med Chem Lett . 2014 Jan 1;24(1):344–8.

12. Aepfelbacher, M. Bacterial lipopolysaccharide up-regulates platelet-activating factor-stimulated Ca2+ mobilization and eicosanoid release in human Mono Mac 6 cells / M. Aepfelbacher, H. W. Ziegler-Heitbrock, I. Lux, P. C. Weber // J Immunol . 1992 Apr 1;148(7):2186–93.

13. Wachowicz, B. Response of blood platelets to Proteus mirabilis lipopolysaccharide / B. Wachowicz, J. Saluk, W. Kaca //Microbiol Immunol . 1998;42(1):47–9.

14. Tuominen, O.-M. Effects of bisphosphonates on prostaglandin E2 and thromboxane B2 production in human whole blood and monocytes stimulated by lipopolysaccharide and A23187 / O.-M. Tuominen, R. Ylitalo-Heikkala, T. I. Vehmas, I. Mucha, P Ylitalo, A Riutta // Methods Find Exp Clin Pharmacol. 2006 Jul-Aug;28(6):361–7

15. Nichols, F. C. Prostaglandin E2, prostaglandin E1 and thromboxane B 2 release from human monocytes treated with C3b or bacterial lipopolysaccharide / F. C. Nichols, H. A. Schenkein, R. B. Rutherford // Biochim Biophys Acta. 1987 Feb 18; 927(2):149–57.

16. Markus Bachschmid Endothelial cell activation by endotoxin involves superoxide/NO-mediated nitration of prostacyclin synthase and thromboxane receptor stimulation / Markus Bachschmid, Svenja Thurau, Ming-Hui Zou, Volker Ullrich //FASEB J. 2003 May;17(8):914–6.

17. Christine Vayssettes-Courchay Role of thromboxane TP and angiotensin AT1 receptors in lipopolysaccharide-induced arterial dysfunction in the rabbit: an in vivo study / Christine Vayssettes-Courchay, Christophe Ragonnet, Gwenolé Camenen, Michel Félétou, Alex A Cordi, Gilbert Lavielle, Tony J Verbeuren // Eur J Pharmacol. 2010 May 25;634(1–3): 113–20.

18. Young, J. M. Human whole blood assays for inhibition of prostaglandin G/H synthases-1 and -2 using A23187 and lipopolysaccharide stimulation of thromboxane B2 production / J. M. Young, S. Panah, C. Satchawatcharaphong, P. S. Cheung // Inflamm Res . 1996 May;45(5):246–53.

19. Stefan Kaehler S-ibuprofen effectively inhibits thromboxane B2 levels and platelet function in an experimental model of lipopolysaccharide-stimulated and non-stimulated whole blood / Stefan Kaehler, Claudia Marsik, Birgit Heinisch, Christiane Thallinger, Robert Sauermann, Lili Kazemi-Shirazi, Oswald Wagner, Christian Joukhadar // Pharmacology. 2008;81(2):181–6.

20. Mayer, A. M. Escherichia coli lipopolysaccharide potentiation and inhibition of rat neonatal microglia superoxide anion generation: correlation with prior lactic dehydrogenase, nitric oxide, tumor necrosis factor-alpha, thromboxane B 2, and metalloprotease release / A. M. Mayer, S. Oh, K. H. Ramsey, P. B. Jacobson, K. B. Glaser, A. M. Romanic // Shock. 1999 Mar;11(3):180–6.